Evaluation of angiosuppressive activity of pleurotus florida on developing chick embryo using chorioallantoic membrane assay

Abstract

Angiogenesis is the proliferation of new blood vessels that enable the normal embryonic development and may also induce growth of numerous pathologies like tumor growth and cancer metastasis. The assays were used for both pro and anti-angiogenic agents. This study evaluated the angiosuppressive activity of Pleurotus florida on the developing chick embryo using chorioallantoic membrane (CAM) assay and used destructive sampling into four different treatment concentrations and a control. The result revealed a decrease in the number of blood vessels branch points formed after 24, 48, and 72 hours at higher treatment concentrations (0.50% and 1%). Statistical analysis showed that 24 hours and 48 hours of exposure to various concentrations have no significant difference. Nevertheless, after 72 hours of exposure, the mean blood vessel branch points formed showed a significant difference among the treatment means between the highest concentration of P. florida (1%) and the control treatment (sterile water). In terms of the percentage of angiosuppressive activity of P. florida with the greatest angiogenic effect, which was the 1% P. florida treatment concentration possesses 35.69% and 36.85% angiosuppressive rate after 48 and 72 hours of exposure respectively. Thus, this study showed that P. florida lyophilized hot water extract exhibits angiosuppressive activity on the developing chick embryo using chorioallantoic membrane assay and is concentration and time exposure dependent.