Lateral bud culture of papaya (Carica papaya) for clonal propagation

Abstract

In vitro culture of lateral buds of field grown mature plants were tested in MS medium supplemented with different concentrations of BAP and NAA. Seasonal endophytic contamination was suppressed by shaking propagules for 2 h in 300 mg L-1 rifampicin before surface sterilization. Maximum survival rate was found in MS medium supplement with 1.0 mg L-1 BAP plus 0.20 mg L-1 NAA. The highest number of shoots was produced in MS medium containing 0.50 mg L-1 BAP and 0.20 mg L-1 NAA. Both the highest multiplication rate and the longest shoot were found in MS medium supplemented with 0.50 mg L-1 BAP plus 0.20 mg L-1 NAA. On the same treatment an increasing trend was observed in multiplication rate of shoot upto 5th subculture which decreased thereafter. First subculture produced the highest shoot length which decreased with the increase in number of subculture. Half strength MS medium supplemented with 1.0 mg L-1 IBA was found as the best treatment to produce root in the culture media. Rooted plantlets were transplanted successfully. © 2007 Asian Network for Scientific Information.