ALPHA AMYLASE INHIBITION ACTIVITY OF OKRA MUCILAGE.

Abstract

In the present study the aqueous seed extract of okra Mucilage was used to examined for alpha amylase inhibition activity. Different concentrations of extracts were incubated with enzyme substrate solution and the activity of enzyme was measured. The maximum inhibition activity was observed at 240 l concentration. The starch agar plate method was also prepared for detection of alpha amylase inhibitor. This inhibitory compound also has insecticidal activity. Diabetes Mellitus is a metabolic disorder characterized by high blood glucose level caused due to deficiency of insulin action. The inhibition of carbohydrate hydrolyzing enzyme such as alpha-amylase can be important strategy in blood glucose level in patients with type II diabetes. In this study male mice were used to measure blood glucose level. The results show that the anti-amylase inhibitor was very powerful to reduce the level of glucose in the blood of the treated mice to the normal comparison with the control.The inhibitory compound was analyzed by FT-IR and SDS-PAGE.Okra Mucilage is an important vegetable. This plant is cultivated in tropical, subtropical and warm temperate region, which is widely distributed from Africa to Asia, Sothern Europe and America. This Okra Mucilage plant belongs to the family Malvaceae, botanical name of this plant is Abelmoschus esculentus.Okra Mucilage plant plays an important role in the human diet by supplying carbohydrates, minerals and vitamins, potassium,sodium,magneshium were found to be the principal element. Okra is a power house of valuable nutrients including fiber,vitamin B 6 and folic acid. Okra is rich in fiber,both soluble and insoluble. Studies counducted at universities of Wisconsin,Madison,USA has revealed that soluble fiber present in Okra helps to lower serum cholesterol,reducing the risk of heart diseases and helps to stabilize blood sugar and binds cholesterol and bile carrying toxic wastes. Whereas, insoluble fiber helps to keep the intestinal tract healthy. Okra is known by many local names in different parts of world.It is called Lady's finger in the England 'Gumbo' in United State of America and 'Bhindi' in India(Chauhan et.al.1972). There are many enzymes in the human digestive system that help in the digestion of food.Alpha amylase catalyses the breakdown of polysaccharide into monosaccharide and only monosaccharide form of food only can absorbed in the stomach.Alpha amylase is an important enzyme that plays an important role in the digestion of starch and glycogen which is present in the saliva and in pancreas of mammals. Alpha amylase inhibitor which inhibit starch hydrolysis which is an important strategy in blood glucose level of Diabetes mellitus. Diabetes mellitus is a