Antioxidant and neuroprotective effects of standardized extracts of Mangifera indica leaf

Abstract

Standardized methanol and aqueous extracts of Mangifera indica L. (mango) leaf were analyzed for antioxidant and neuroprotective activities. Antioxidant activities were evaluated by 2, 2-azinobis 3-ethylbenzothiazoline 6-sulfonate (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide (O•2), hydrogen peroxide (H2O2) scavenging assays and ferric reducing antioxidant power (FRAP) including ion-chelating activity. The neuroprotective effect was evaluated by measuring the protection of neuroblastoma cells from H2O2-induced oxidative injury. The IC50 values for the DPPH of the methanol and aqueous extracts were 6.18 ± 0.15 and 5.57 ± 0.18 μg/ml. The IC50 values for the ABTS of the methanol and aqueous extracts were 1.33 ± 0.13 and 2.96 ± 0.05 μg/ml. The EC1 of FRAP for the methanol and aqueous extracts were 202.92 ± 3.39 and 298.55 ± 8.65 μg/ml, respectively. The IC50 values for the (O•2) scavenging of the methanol and aqueous extracts were 0.07 ± 0.01 and 0.06 ± 0.01 μg/ml. The IC50 values for the H2O2 scavenging and ferrous ion chelating activity of the methanol extract were 70.89 ± 6.56 and 0.89 ± 0.81 μg/ml, respectively. The methanol extracts at 30-50 μg/ml and aqueous extracts at 100 μg/ml as well as mangiferin, a biologically active component present in the leaf of M. indica, at 100 μM effectively protected neuroblastoma cells from H2O2-induced oxidative damage. The beneficial effects of these extracts appear to be promising for neuroprotection.