Erratum: Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases (PLoS ONE (2013) 8:4 (e62230) DOI: 10.1371/journal.pone.0062230)

Abstract

Following the publication of this article [1], concerns were raised regarding results presented in Figs 4 and 6. Specifically, • The following Fig 4B panels appear similar: CDC42 biosensors WB FC-Rho, CDC42 biosensors CoIP FN-WASP when rotated 180°, and RhoA biosensors CoIP FN-PAK when flipped horizontally • The following Fig 4B panels appear to partially overlap with one another: Rac1 biosensors WB FC-Rho, RhoA biosensors WB FC-Rho, and RhoA biosensors WB GAPDH when rotated 180° • The following Fig 6A panels appear similar: Active RhoA, active Rac1, and active CDC42 when rotated 180° and vertically compressed The corresponding author commented that errors were made during figure preparation, due to the similarities in bands and overall underlying images. The corresponding author also clarified that during their post-publication review of the data, they noticed that the following published panels were also incorrect: • Fig 4B CDC42 biosensors WB FN-WASP • Fig 4B Rac1 biosensors CoIP FN-PKN • Fig 4B RhoA biosensors WB FN-PAK The corresponding author provided the original underlying data for the blots presented in Figs 4B and 6A, published in the S1 and S2 Files available with this notice, as well as the updated Figs 4B and 6A below. (Figure Presented) The authors did not comment on the availability of the remaining data presented in this study. The provided data and updated figures support the findings reported in [1], but the PLOS ONE Editors remain concerned about the number of panels affected by data reporting issues. We issue this Expression of Concern to notify readers of the above concerns and to relay the available data provided by the corresponding author.