Miracidia as main source for autofluorescence of Schistosoma mansoni eggs

Abstract

BACKGROUND: Egg detection still has a role in schistosomiasis control, as a screening strategy or to provide a reference standard for the assessment of the accuracy of other diagnostic tools. The Helmintex method is highly sensitive but laborious, and several improvements of it, including automated egg detection, are currently under development. OBJECTIVE: We conducted a preliminary evaluation of Schistosoma mansoni eggs’ autofluorescence as a distinctive marker amid very complex fecal sediments. METHOD: Eggs from mouse livers and human feces were examined under a fluorescence microscope. FINDINGS: More intense green fluorescence (greater for miracidia than for eggshell) was consistently detected using a B-2A filter (FITC, 420–495 nm). MAIN CONCLUSIONS: These findings may help to improve diagnostic methods, especially with automated egg detection systems. Besides access to safe water and adequate sanitation, as well as health education and the treatment of infected individuals, laboratory diagnosis is a key measure that can help eliminate schistosomiasis as a public health problem.