Inhibition of Ca2+ conductance in identified leech neurons by benzodiazepines

Abstract

Benzodiazepines (BZs) in micromolar concentrations inhibit Mn2+ - and Co2+ -sensitive regenerative divalent cation potentials, which are revealed in the presence of tetraethylammonium ion, in leech nociceptive neurons (N cells). This BZ effect is reversible and dose-dependent. The BZs, like Mn2+ and Co2+, inhibit the maximum rate of depolarization (̇V(max)) and duration of divalent cation potentials at concentrations that do not significantly affect resting membrane potential or ̇V(max) of the Na+ -dependent action potential. Ultraviolet-induced BZ binding to micromolar-affinity sites in ganglia and isolated cells irreversibly blocks Ca2+ conductance in neurons without significantly affecting resting membrane potentials. BZ binding studies with leech neuronal membrane show saturable, specific binding in the micromolar concentration range that was similar to BZ binding to synaptosomal membrane fractions. The apparent (KD) obtained from the micromolar-affinity BZ binding curve for leech ganglionic membrane preparations agrees well with the apparent (Ki) estimated from the dose-response curve measuring BZ inhibition of (̇Vmax) of the divalent cation potentials. These findings indicate that BZs act like Ca2+ -channel antagonists in intact neuronal preparations and are consistent with the hypothesis that BZ binding to micromolar-affinity receptors modulates voltage-gated CA2+ channels.