Effect of Percoll Density Gradient Centrifugation on Semen Quality of X-Sperm Enriched Crossbred Bull Semen

Abstract

The present study was conducted to observe the effect of percoll density gradient method on X-sperm enrichment and quality of semen. It was performed at Semen Production Centre, CVASc, GBPUAT, Pantnagar, Uttarakhand. Ejaculates (n=6) were collected by AV method from Crossbred bulls. X-sperm enrichment was done by percoll density gradient method i.e. 7 layers (70-10%). One ml semen was placed over the gradient in conical centrifuge tube. Centrifugation was done at 750g (22-24°C) for 15 min. The pH of percoll density gradient centrifuged (supernatant) decreased significantly (P<0.05) in Crossbred semen. Progressive motility of percoll density gradient centrifuged (supernatant) decreased significantly (P<0.05) in Crossbred cattle semen. Live spermatozoa (%) of enriched semen (pellet) were increased in Crossbred cattle semen. Acrosome integrity was not affected in all the groups in Crossbred bull. HOST responsive sperm were not affected in all the groups of Crossbred cattle semen. Thus, percoll density gradient centrifugation (7 layer 70%) did not affect the semen quality and fertility of semen hence it may be used to increase birth of female calves using A.I.