UniFrag and GenomePrimer: Selection of primers for genome-wide production of unique amplicons

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Abstract

Summary: the complementary programs UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility.

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van Hijum, S. A. F. T., de Jong, A., Buist, G., Kok, J., & Kuipers, O. P. (2003). UniFrag and GenomePrimer: Selection of primers for genome-wide production of unique amplicons. Bioinformatics, 19(12), 1580–1582. https://doi.org/10.1093/bioinformatics/btg203

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