Analyses of conformational states of the transporter associated with antigen processing (TAP) protein in a native cellular membrane environment

Abstract

Background: TAP proteins tagged with CFP and YFP were used for FRET spectroscopy measurements. Results: Conformational changes of the nucleotide binding domains (NBD) were measurable in permeabilized cells. Conclusion: TAP-specific peptides induce NBD closure, and maximal NBD closure is induced by the combination of a peptide and a non-hydrolysable ATP analog. Significance: These studies elucidate distinct steps of the TAP transport cycle. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.