Influence of auxin concentration on in vitro rooting of chrysanthemum morifolium Ramat

Abstract

Efficient in vitro rooting of Chrysanthemum morifolium Ramat., using hormone free media with different concentrations of auxins was developed. In this study we used three different auxins at various concentrations (0, 0.1, 0.5 and 1.0 mg/L) of IAA (Indole-3-acetic acid), IBA (Indole-3-butyric acid) and NAA (1-Naphthaleneacetic acid) for root development in Chrysanthemum morifolium. Shoot explants from in vitro culture were grown on a medium to determine the potential of rooting frequency, number of roots per explant and root length. However, SH medium with 1mg/L of (IBA) induced the highest number 5.7 ± 0.5 of root per explant among the three different concentrations of auxin used followed by (IAA) and NAA. The highest root length 36.2 ± 3.3 was achieved in SH medium with 1mg/L of IAA and lesser number of roots and root length were developed in NAA. The in vitro rooted plants were hardened and acclimatized in the greenhouse and successfully (100%) transplanted to the field. Protocols were developed in the present investigation for cloning and large scale plant production of Chrysanthemum (C. morifolium Ramat.).