Simultaneous determination of ten antibiotics in natural water samples by capillary electrophoresis with electrochemiluminescence detection coupled with hollow fiber-Solid phase extraction

Abstract

A novel method for simultaneous determination of ten antibiotics in natural water samples was established based on purifing and enriching by hollow fiber (HF) solid phase extraction (SPE). separating by capillary electrophoresis (CE) and detecting by electrochemiluminescence (ECL). The effects of water sample pH, extraction time and extraction temperature on the extraction efficiency of ten antibiotics were studied. The optimized parameters of SPE with 0.2 g HF were determined as follows: water sample pH 6. extraction time 40 min and extraction temperature 50°C. The effects of separation buffer solution pH, ionic strength, separation voltage and additives on the separation efficiency often antibiotics were investigated. The selected CE separation solution is phosphate buffer solution (pH 6.5) containing 50 mmol/L NaCl and 35 mmol/L isopropanol. The separation voltage is 19 kV. All the ten antibiotics have good linear relationship. Their detection limits were 0.4-1.2 (. ig/L. The recoveries of water samples were 81.6%-115.7%. The method is sensitive, accurate and rapid, and can be used to determine antibiotics in natural water samples directly.