Immunizing properties of insoluble cell material derived from brucella

Abstract

AN insoluble fraction was obtained by high-speed centrifugation from Brucella suis, after disintegration in a sonic vibrator1. This fraction could absorb the agglutinating and mouse protecting antibodies from B. suis antisera. Since similar absorptions could be carried out, although less effectively, with whole bacteria, the insoluble material was assumed to be situated on the cellular surface and tentatively regarded as part of the cell wall. © 1959 Nature Publishing Group.