Determination of ropivacaine and bupivacaine in human plasma by programmed temperature vaporiser-fast gas chromatography-mass spectrometry (PTV/fast GC/MS) utilising in-vial liquid-liquid extraction

Abstract

In-vial liquid-liquid extraction has been investigated as a sample-preparation method in combination with fast gas chromatography and mass spectrometric detection for analysis of ropivacaine and bupivacaine in human plasma. The method is simple, quick, and readily automated. It offers a new possibility for the GC analysis of drugs in biological fluids. Validation showed that the method is accurate and selective. The acceptance criteria for the study validation were well in line with international criteria.