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Fluorescent labeling of bacteriophage T7 by CRISPR-Cas9

Journal of Physics: Conference Series (2019) 1400(3)
DOI: 10.1088/1742-6596/1400/3/033005
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Abstract

The objective of this work was to develop a simple method for coupling a fluorescent protein with a bacteriophage capsid. The Escherichia coli bacteriophage T7 was chosen as a model one. The method, based on CRISPR-Cas9 system and the λ-Red recombination, allowed to insert the gene of enhanced yellow fluorescent protein into the phage genome.

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APA

Znobishcheva, E. A., Morozova, N. E., & Khodorkovskii, M. A. (2019). Fluorescent labeling of bacteriophage T7 by CRISPR-Cas9. In Journal of Physics: Conference Series (Vol. 1400). Institute of Physics Publishing. https://doi.org/10.1088/1742-6596/1400/3/033005

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