The Effect of Roselle (Hibiscus sabdariffa Linn) Flower Extract to the SGPT Activity in Male Wistar Rats (Rattus Norvegicus) Induced by High Dose Paracetamol

Abstract

Paracetamol is an analgesic drug that is used widely. The use of high dose paracetamol can cause liver damage. Based on research, Roselle has high antioxidant content.The objective of the research was to find out the effect of Roselle (Hibiscus sabdariffa Linn) flower extract to the SGPT activity of Wistar rats induced by high dose paracetamol. This study used 24 male Wistar rats as samples and conducted for 10 days. Samples were divided into 3 groups : Group of rats fed with standard diet and filtered water for 10 days and received 0.5% CMC-Na orally on the 9 th day; group of rats fed with standard diet filtered water for 10 days and received paracetamol 1750mg/kg body weight (in 0.5% CMC-Na) orally on the 9 th day; group of rats fed with standard diet and Roselle flower extract 200mg/kg body weight for 10 days and received paracetamol 1750mg/kg body weight (in 0.5% CMC-Na) orally on the 9 th day. All group were sacrified on the 10 th day. SGPT activity were examinated with spectrophotometry method.The result of Kruskal-Wallis test showed significant difference (p=0.001) of SGPT activity between the group of rats received standard diet (81,44 U/L) and group of rats received high dose paracetamol (240,39 U/L). Furthermore, there is significant difference (p=0.001) of SGPT activity between group of rats received high dose paracetamol (240,39 U/L) and group of rats received high dose parasetamol and Roselle flower extract (72,98 U/L).It can be concluded that high dose of paracetamol significantly increased SGPT activity, and Roselle flower extract significantly reduced SGOT activity of Wistar rats induced by high dose parasetamol.