The karyotypes of three Tuber species (Pezizales, Ascomycota)

Abstract

The present work investigates the karyotypes of Tuber borchii Vittad., Tuber magnatum Pico and Tuber melanosporum Vittad. The chromosome diploid numbers were 8 for the three species examined as determined in metaphase configurations from hyphae nuclei by the squash method and aceto-orcein staining. We investigated the three species by means of an image analysis system to obtain measurements of the chromosomes. Range of chromosome length was from 1.26 μm the longest in T. melanosporum, to 0.65 μm, the shortest in T. magnatum. © 2002 Taylor and Francis Group, LLC.