Indica-Japonica differentiation of paddy rice and its relationship with heterosis

Abstract

The Indica-Japonica differentiation of three photoperiod-sensitive and/or thermosensitive genetic male-sterile rice (PGMS or TGMS, respectively) lines and 47 male parental lines from seven ecotypes were studied for their restriction fragment length polymorphism marker data to determine which ecotype crosses with the three PGMS and/or TGMS lines could lead to higher yield potential, and to estimate the relationship between the Indica-Japonica differentiation of parents and heterosis in grain yield and its components. The results indicated that hybrids derived from 'N422s' and the early-middle ripening Indica varieties from southern China, and hybrids between 'Pei'ai64s' and three Japonica ecotypes, including North-eastern Japonica varieties, restoring lines of Japonica hybrid rice and north China Japonica varieties, showed the highest grain yields. There was less variation of yield among the F1s between '108s' and the seven ecotypes than among the other F1s. Highly significant positive correlations between heterosis of the F1 yield and genetic distance of the parents were detected, although the correlation between F1 yield performance and genetic distance did not reach a significant level. Considerable variation of correlation between heterosis and genetic distance was also detected in the Indica × Indica crosses and Indica × Japonica crosses. There was much higher correlation (r = 0.63) between the F1 yield performance and the genetic distance of parents in the Indica × Indica crosses than in the others. It is proposed that a genetic distance of 0.4-0.8 between the two parents of hybrid rice might be appropriate not only for F1 performance, but also for heterosis.