Cuantificación de Sapogeninas del Jugo Fresco y Fermentado de Fique (Furcraea gigantea) mediante Cromatografía Liquida de Alta Resolución (HPLC-PDA)

Abstract

A study on the quantification of sapogenins in fresh and fermented juice of fique (Furcraea gigantean) by high-performance liquid chromatography with photodiode array detector (HPLCPDA). The juice of the fique, a plant that is widely grown in Colombia, was fermented for 2, 4 and 6 days at 33 °C. Using acid hydrolysis, sapogenin extracts were obtained. The substances in the extracts, identified by gas chromatography-mass spectrometry (GC-MS) were hecogenin and tigogenin. Hecogenin and tigogenin in the juice were determined using HPLC-PDA. Fique juice with four days of fermentation showed the highest content of sapogenins. Therefore, this is the recommend process for the extraction of these compounds.