Activity of Guaiacol Peroxidase of Solanum Lycopersicum In presence of Detergents and Chaotropic Agents

Abstract

Browning of damaged tissues in fresh fruits and vegetables results mainly from the oxidation of phenolic compounds to quinones by peroxidase. The activation of Peroxidase by sodium dodesyl sulphat investigated, but reports on the effect of detergents and chaotropic agents on activity of Peroxidase are scarce. Here the effect of some detergents and chaotropic agent on activity of enzyme in soluble and in partial purified fraction was investigated. Sarkosyl inhibited activity of enzyme for oxidation of guaiacol and H2O2, although has inhibitory effect in higher concentrations. Other ionic, Nonionic detergents and Chaotropic agents showed inhibitory effect on activity of Peroxidasein partial purified fraction. Inhibitory effect of Urea, GnHcl, NP-40, Triton x-100 and Sodium cholate in presence of these substrates investigated so GnHcl and NP40 showed that they are potent inhibitor of peroxidase and sodium cholate showed that it is a weak inhibitor of guaiacol peroxidase. Results identified and confirmed the differences in structure and conformation of enzyme in soluble and in partial purified fraction for oxidation of different substrates.