In vitro incorporation of fucose and methionine into human platelet proteins

Abstract

Human blood platelets, maintained in vitro, incorporate labelled methionine into a variety of high molecular weight proteins and labelled fucose into a different set of glycoproteins. The incorporation of methionine is completely sensitive to cycloheximide and partially sensitive to chloramphenicol and leucovorin demonstrating de novo synthesis. The incorporation of fucose is insensitive to these inhibitors but inhibited by 6-thioguanine which would indicate that a fucosyl transferase is adding the sugar onto pre-existing glycoproteins. Some of the fucosyl-glycoproteins correspond to surface glycoproteins. © 1982.