Optimization of Enzymatic Saccharification of Alkali Pretreated Typha angustifolia for Glucose Production

Abstract

Lignocellulose ethanol is significantly sustainable bio-fuel. It is environmentally friendly. This work is to develop a hydrolysis process of pretreated lignocellulose to ethanol prodn. from narrow leaved cattail as a biomass material. Response surface methodol. (RSM) with a central composite design (CCD) was followed to optimize the enzymic saccharification process in order to obtain high glucose yield. Three independent variables (cellulase, $β$-glucosidase and temp.) that operating condition, vary at CCD five levels (-$α$, -1, 0, +1, +$α$). The optimum values for the predicted variables for glucose and xylose released were: cellulase loading 17.5 FPU/g substrate, $β$-glucosidase loading 0 U/g substrate and hydrolysis temp. 55°C. Under these optimal conditions, glucose and xylose yield reached to 413.25 mg/ g substrate and 75.48 mg/g substrate, resp. The results of a confirmation expt. under the optimum conditions agreed with model predictions. [on SciFinder(R)]