Microfluidic barcode assay for multiplexed clinical diagnostics

Abstract

Here we present a novel method for creating three-dimensional, photocaptured protein patterns that can be completed with no additional linker molecules required. We utilized the photopatterned proteins to selectively capture antibodies and implemented a microfluidic sandwich assay. We demonstrate Hepatitis C virus (HCV) diagnostic device based on the technology and show that the device is capable of assaying human sera and can positively identify HCV+ human patient serum samples in 60 minutes. We also demonstrate a barcode assay using lectins to detect differences in prostate specific antigen (PSA) glycan structure. Additionally, we image the HCV diagnostic chip using a low-resource setting compatible imagining system – the fluorescence CellScope.