Improving rate of success in isolating Haemophilus parasuis from clinical samples

Abstract

The rate of success in isolating H parasuis from clinical samples may be improved by following a few critical procedures. As this organism is very sensitive to high temperatures, samples should always be maintained under refrigeration. Transport systems containing Amies medium or submission of whole tissue tend to improve viability of H parasuis during transportation. It is important to collect samples from untreated, acutely infected pigs. Haemophilus parasuis is more likely to be isolated from pigs that have been euthanized than from dead pigs.