Induction of tolerance to desiccation and to subzero temperatures in embryos of recalcitrant seeds of inga1

Abstract

Drying, widely used for storing orthodox seeds for prolonged periods, cannot be applied to recalcitrant seeds, which are sensitive to desiccation. Thus, inclusion of species with recalcitrant seeds, like inga, in reforestation programs or even for commercial use has been hindered by the lack of technology that would allow storage of these seeds. The remaining option, cryopreservation, is a method of high cost that requires a high level of technology. Knowledge of the processes involved in sensitivity to desiccation continues to be a great challenge for the seed sector. The aim of this study was to analyze the effects of osmotic treatments on tolerance to desiccation and storage capacity of recalcitrant seeds of inga. Embryos were subjected to osmotic stresses with PEG solution and subjected to progressive drying processes. In another experiment, the effects of these solutions on embryo conservation during storage were analyzed. From the results, it may be concluded that incubation of embryos in a solution with -2.0 MPa increases their tolerance to desiccation.