Effect of temperature on the concentration of Chrysanthemum stunt viroid in CSVd-infected chrysanthemum

Abstract

CSVd-infected chrysanthemum plants grown under 10°C or 15°C growth chamber for 2 months resulted a higher dilution endpoint of template RNA for Reverse transcription and polymerase chain reaction (RT-PCR) than those grown for 1 month: 10-4 (1.35×10-2 ug/ml) for 1 month, and 103 (1.35×10-1 ug/ml) for 2 months. Independent experiment, shoots cut from CSVd (Chrysanthemum stunt viroid)-infected chrysanthemum plants grown under 10°C or 20°C growth chamber for 2 months showed the same CSVd concentration as control (30°C) at 8 weeks after moving them to normal green-house condition (30°C). From those results, it was concluded that even though the concentration of CSVd was reduced in plants grown at low temperatures, when they were moved to normal glass-house temperature CSVd concentration increased to that of untreated plants in 8 weeks. This conclusion was supported by the rapid replication of CSVd in chrysanthemum after infection. © The Korean Society of Plant Pathology.