Chloroplast DNA variation and hybridization between invasive populations of Japanese knotweed and giant knotweed (Fallopia, Polygonaceae)

Abstract

Chloroplast inheritance and the direction of hybridization have been investigated in some invasive weeds from the genus Fallopia. PCR RFLP analysis of the trak intron has been used to identify markers that distinguish between the chloroplast genomes of British Samples of E japonica vat. japonica, F. japonica var. compacta and F. sachalinensis. Maternal inheritance of chloroplast DNA has been observed in controlled crosses and in hybrid seed from known maternal parents. PCR RFLP analysis of wild hybrids between E japonica var. japonica and either F. japonica var. compacta or F. sachalinensis indicates that F. japonica var. japonica is the maternal parent of all of its hybrids, consistent with the apparent male-sterility of this taxon in Britain. Hybrids between F. japonica var. compacta and F. sachalinensis show the chloroplast haplotype of one or other of the parents, consistent with bi-directional hybridization.