Utilization of a novel Ag(III)-luminol chemiluminescence system for determination of d-penicillamine in human urine samples

Abstract

A novel sensitive chemiluminescence (CL) system ([Ag(HIO6) 2]5--luminol reaction system), coupled with a flow injection, is introduced for determination of d-penicillamine. The system is based on the enhancement effect of chemiluminescence reaction by penicillamine in alkaline medium. Under the optimum conditions, the CL intensity is proportional to the concentrations of d-penicillamine with a good linearity. The linear range covers 1.5-150 ng mL-1 and the limit of detection of the method is 0.8 ng mL-1. The relative standard deviation is 0.23% for 11 repeated measurements of 0.06 μg mL-1 d-penicillamine. The method has been successfully applied to the determinations of d-penicillamine in pharmaceutical preparations and urine samples, giving rise to satisfactory results. Based on the chemiluminescent spectra and free radical trapping experiment performed in this work, a possible reaction mechanism for this reaction system is suggested. © 2011 Sociedade Brasileira de Química.