Establishment of primary mouse lung adenocarcinoma cell culture

Abstract

Lung cancer is the most common malignant disease worldwide and is the leading cause of death from cancer. Primary cultures derived from lung cancer are essential for understanding abnormal growth function in lung epithelia. In this study, 2 out of 5 primary lung adenocarcinoma cultures derived from DNA repair-deficient mice were established and characterised using electron microscopy and immunostaining. Results of the tumourigenicity tests confirmed that these primary cells are tumourigenic. In conclusion, an effective primary culture method provides a tool for clinical antitumour drug testing.