PLGA+Fe3 O4 +PFP Nanoparticles Drug-Delivery Demonstrates Potential Anti-Tumor Effects on Tumor Cells

Abstract

Background: Material/Methods: Results: Conclusions: Nanoparticles are proven as a potential tool for treating various disorders. However, efficient nanoparticle delivery of antitumor drugs is urgently needed for tumor treatment. This study aimed to generate a drug-delivery nanoparticle with higher efficacy and safety. We developed a poly-(lactide-co-glycolide) (PLGA) nanoparticle (FLGA-Fe3 O4 +PFP) embedded with super-para-magnetic iron oxide (Fe3 O4 ) and perfluoropentane (PFP). Characteristics of FLGA-Fe3 O4 +PFP nanoparticles were observed using optical microscopy, scanning electron microscopy, and transmission electron microscopy. HNE1 and HepG2 cells were cultured and used for experiments. MTT was used to evaluate cytotoxic effects of FLGA-Fe3 O4 +PFP nanoparticles on HNE1 and HepG2 cells. Cell engulfment capacity was examined and a cell targeting experiment was conducted to evaluate invasive capability and binding efficiency of PLGA+Fe3 O4 +PFP nanoparticles, respectively. Biological toxicity of PLGA+Fe3 O4 +PFP nanoparticles in rats was evaluated by determining CK, LDH, creatinine, and UA levels, and ALT and AST activities. PLGA+Fe3 O4 +PFP nanoparticles demonstrated well-defined spherical and dispersed morphology with smooth surfaces. There were scattered black spots on shells of PLGA+Fe3 O4 +PFP nanoparticles. PLGA+Fe3 O4 +PFP nanoparticles did not trigger obvious effects on cell viability of HNE1 and HepG2 cells. HNE1 and HepG2 cells demonstrated higher engulfment capacity for PLGA+ Fe3 O4 +PFP nanoparticles. PLGA+Fe3 O4 +PFP nanoparticles demonstrated higher targeting CDDP delivery efficacy and promoted binding efficiency of targeting CDDP with cells. PLGA+Fe3 O4 +PFP nanoparticles demonstrated no obvious toxic effects on heart, kidney, liver (without effects on CK, LDH, creatinine, UA levels, and ALT and AST activities). PLGA+Fe3 O4 +PFP nanoparticles were safe, with higher invasive ability and binding efficiency of targeting CDDP with tumor cells. Therefore, PLGA+Fe3 O4 +PFP nanoparticles demonstrated potential anti-tumor effects after transplantation.