LC/MS/MS warfarin assay - An emerging tool for the early detection of cytochrome P450-associated drug - drug interactions in drug discovery

Abstract

The LC/MS/MS warfarin assay, combining stereo- and regioselective cytochrome P450 (CYP) form-specific warfarin hydroxylation with sensitive and specific LC/MS/MS detection technique, is emerging to be a promising tool for the study of CYP-associated drug - drug interactions for new chemical entities (NCEs) during drug discovery process.