Optimization of lipase synthesis by pseudomonas fluorescens by response surface methodology

Abstract

Using response surface methodology (RSM), the optimum environmental conditions of temperature, pH. and aeration for lipase synthesis by Pseudomonas fluorescens were found to be 25°C, 8.07, and 65 rpm, respectively, for 5 d of incubation. Of the three factors, temperature was most influential in affecting extracellular lipase production.