Improving the osteoblast cell adhesion on electron beam controlled tionanotubes

Abstract

Here we investigate the osteogenesis and synostosis processes on the surface-modified TiOnanotubes via electron beam irradiation. The TiOnanotubes studied were synthesized by anodization process under different anodizing voltage. For the anodization voltage of 15, 20, and 25 V, TiOnanotubes with diameters of 59, 82, and 105 nm and length of 115, 276, and 310 nm were obtained, respectively. MC3T3-E1 osteoblast cell line was incubated on the TiOnanotubes to monitor the change in the cell adhesion before and after the electron beam irradiation. We observe that the electron beam irradiation affects the number of surviving osteoblast cells as well as the cultivation time. In particular, the high adhesion rate of 155% was obtained when the osteoblast cells were cultivated for 2 hours on the TiOnanotube, anodized under 20 V, and irradiated with 5,000 kGy of electron beam. © 2014 Sung Wook Yoon et al.