SECONDARY METABOLITE FROM MANGROVE ENDOPHYTIC FUNGUS Fusarium proliferatum AED3

Abstract

As part of an ongoing search for secondary metabolites from an endophytic fungus Fusarium proliferatum AED3 derived from the mangrove plant Ardisia elliptica, a bioactive compound, was isolated by bioactivity-guided fractionation. The structure of the isolated compound was determined based on nuclear magnetic resonance spectroscopy and mass spectrometric data (UV, IR, LC-MS/MS, and NMR). The cytotoxic activity of the isolated compound was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against the T47D cancer cell line. Based on spectroscopic data, it was concluded that the isolated compound was beauvericin (1). This compound exhibited mild cytotoxic activity against the T47D cancer cell line with an IC50 value of 112.2 μg/mL.