Green method for act and ibuprofen simultaneous assay in the combination tablet using FTIR

Abstract

Objective: The purpose of this study was to develop a green method for assay the tablet consists of a combination of acetaminophen (ACT)-ibuprofen (IBU). FTIR (Fourier Transform Infrared Spectroscopy), which is commonly used for qualitative analysis, was developed to measure the tablet contents, directly and quantitatively. Methods: Standard for ACT and IBU was respectively mixed with KBr crystal in varied and measured using FTIR. The transmission spectrum yielded later was converted into its absorbance-derivatized forms which then a calibration curve was composed using the data. Once validated, the analytical method was conducted on samples of the branded ACT-IBU combination tablet. Results: ACT had a specific wavenumber of a group N-H stretch, meanwhile IBU was represented by the C = O spectrum. These peaks then used for quantitative calculation basis levels respectively. The limits of detection and quantitation of ACT consecutively were 3.249 × 10-5 % w/w and 1.083 × 10-4 % w/w and IBU were 6.6652 × 10-4 % w/w and 2.2174 × 10-3 % w/w. Next; the method was carried out successfully to evaluate the content of sample tablet from the market. Conclusion: The analytical method of ACT-IBU was proven applicable and suitable for the quantitative purpose. The method shows meet with the expectation such as simple and easy to perform and reduce the use of solvents.