Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding

Abstract

The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described. © 2006 International Union of Crystallography. All rights reserved.