Binding of Human Carbonic Anhydrase to Human Hemoglobin

Abstract

The ability of human carbonic anhydrases to interact with human CO‐hemoglobin have been studied with the counter‐current distribution technique in aqueous/aqueous biphasic systems. The experimental results show that human carbonic anhydrase II interacts with human CO‐hemoglobin whereas human carbonic anhydrase I does not. The interaction between CO‐hemoglobin and carbonic anhydrase II was quantified using the theoretical model developed previously for one‐to‐one interacting systems [Backman, L. and Shanbhag, V.P. (1979) J.Chromatogr, 171, 1–13]. The apparent association constant was estimated to be 4.1×105 1 mol−1 at pH 8.0 and 21 °C for the association of carbonic anhydrase II and CO‐hemoglobin. Copyright © 1981, Wiley Blackwell. All rights reserved