Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP

Abstract

Intimin is a highly polymorphic protein encoded by the eae gene and plays a crucial role in the attaching-effacing phenotype of diarrheagenic Escherichia coli and related pathogens. We have developed a method to quickly and accurately uncover allelic variation at the eae locus through the use of fluorescent RFLP (fRFLP). Application of fRFLP to 151 eae-positive strains (including the newly described Escherichia albertii) revealed 26 different fRFLP types that correspond to 20 of the 28 previously described eae alleles. Two sequence variants of the γ, 1, κ, and ζ alleles and three variants of ε were also observed. In addition to being reliable and accurate, the method can be easily adapted to accommodate new eae allelic sequences, as they become known. © 2006 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.