Mutation conferring apical-targeting motif on AE1 exchanger causes autosomal dominant distal RTA

Abstract

Mutations in SLC4A1 that mislocalize its product, the chloride/bicarbonate exchanger AE1, away fromits normal position on the basolateral membrane of the a-intercalated cell cause autosomal dominant distal renal tubular acidosis (dRTA).We studied a family exhibiting dominant inheritance and defined amutation (AE1-M909T) that affects the C terminus of AE1, a region rich in potential targeting motifs that are incompletely characterized. Expression of AE1-M909T in Xenopus oocytes confirmed preservation of its anion exchange function.Wild-type GFP-tagged AE1 localized to the basolateral membrane of polarized MDCK cells, but AE1-M909T localized to both the apical and basolateral membranes. Wild-type AE1 trafficked directly to the basolateral membrane without apical passage, whereas AE1-M909T trafficked to both cell surfaces, implying the gain of an apical-targeting signal.We found that AE1-M909T acquired class 1 PDZ ligand activity that the wild type did not possess. In summary, the AE1-M909T mutation illustrates the role of abnormal targeting in dRTA and provides insight into C-terminal motifs that govern normal trafficking of AE1. Copyright © 2012 by the American Society of Nephrology.