Molecular Mechanisms and Signaling Pathways Underlying the Therapeutic Potential of Thymoquinone Against Colorectal Cancer

Abstract

Thymoquinone (TQ), a bioactive compound derived from Nigella sativa, has garnered significant attention for its potential as a natural anti-cancer agent, particularly in the context of colorectal cancer. This review provides a detailed synthesis of the current literature on the anti-cancer properties of TQ in colorectal cancer cells, exploring both in vitro and in vivo studies to elucidate its mechanisms of action. TQ effectively induces apoptosis, inhibits cell proliferation, and reduces metastasis in colorectal cancer cells by modulating key molecular pathways such as PI3K/AKT/mTOR, NF-κB, STAT3, and MAPK. It causes mitochondrial dysfunction and activates caspases, contributing to its pro-apoptotic effects. TQ also regulates EMT and targets cancer stem cells, reducing the likelihood of metastasis. Moreover, its antioxidant properties contribute to its protective role against cancer progression. While preclinical studies provide strong evidence of TQ’s efficacy, further clinical studies are essential to establish its therapeutic potential in humans. This review underscores TQ’s promising role as a natural agent with the potential to significantly improve colorectal cancer treatment outcomes.