Immunohistochemical localization of thyrotropin-releasing hormone in the rat hypothalamus and pituitary

268Citations
Citations of this article
20Readers
Mendeley users who have this article in their library.

Abstract

The distribution of immunoreactive TRH in the rat hypothalamus and pituitary was demonstrated using the peroxidase-antiperoxidase technique after rapid fixation of the rat brain with 5% acrolein. Widespread reaction product was identified in neuronal processes throughout the hypothalmus, with dense labeling in the median eminence, dorsomedial nucleus, parvocellular division of the paraventricular nucleus, perifornical region, periventricular nucleus, and organum vasculosum of the lamina terminalis. A striking accumulation of immunoreactive TRH was also noted throughout the posterior pituitary, where fibers appeared to terminate in grape-like swellings. Peroxidase-positive perikarya were best seen after colchicine pretreatment and were distributed in many regions of the hypothalamus. The greatest density of immunoreactive neurons was in the suprachiasmatic preoptic nucleus, parvocellular subdivision of the paraventricular nucleus, perifornical region, dorsomedial nucleus, and baso-lateral hypothalamus. These data are consistent with the role of TRH as a hypophysiotropic hormone, a regulator of the posterior pituitary, and a neurotransmitter or neuromodulator of neurons in other regions of the hypothalmus. © 1982 by The Endocrine Society.

Cite

CITATION STYLE

APA

Lechan, R. M., & Jackson, I. M. D. (1982). Immunohistochemical localization of thyrotropin-releasing hormone in the rat hypothalamus and pituitary. Endocrinology, 111(1), 55–65. https://doi.org/10.1210/endo-111-1-55

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free