Inhibitory effect of TNF-α and IL-8 secretion by pimarane-type diterpenoids from acanthopanax koreanum

Abstract

A new pimarane-type diterpene compound, acanthokoreoic acid A together with three known compounds, acanthoic acid, acanthol, and sumogaside were isolated from a CH2Cl2 fraction of Acanthopanax koreanum by repeated column chromatography and reversed phase preparative HPLC. Acanthoic acid was isolated in high yields and showed potent inhibitory activity on the IL-8 secretion of the TNF-α-stimulated human colon adenocarcinoma cell line HT-29 and on the TNF-α secretion of the trypsin-stimulated human leukemic mast cell line HMC-1. © 2003 Pharmaceutical Society of Japan.