OPTIMIZATION OF PAPAIN SOLUBLE COLLAGEN EXTRACTION FROM MILKFISH SCALES (CHANOS CHANOS FORSKAL)

Abstract

This study aims to determine the papain soluble collagen from the milkfish scale based on the yield and antioxidant activity due to treatment of extraction time and papain concentration. Papain soluble collagen (PSC) was isolated from the scales of milkfish (Chanos chanos) caught in Sidoarjo, Indonesia, with a total production of 828,372.62 tons in 2018. Independent variables of extraction time were (X1, hour) and papain concentration (X2, U/mg). Dependent variables were yield (Y1, %) and antioxidant activity (Y2, mg/L).This research used Response Surface Methodology. The result showed that the extraction time and papain concentration from 24 hours to 82 hours with papain concentration 3000 U/mg to 7000 U/mg resulted in yield 2.25% to 6.4% and antioxidant activity of 199 mg/L to 418 mg/L. The optimal condition of extraction PSC was 56.45 hours with papain concentration 5950 U/mg resulting in a yield of 6, 0%±0,012 and antioxidant activity of 258±0, 98 mg/L.