Establishment of the Shoot and Callus Culture of an Important Medicinal Plant Plumbago zeylanica

Abstract

Plumbago zeylanica is an important medicinal plant which belongs to the Plumbaginaceae family. It possesses antibacterial, hepato protective, and central nervous system stimulatory activity, anti-fungal, anti-inflammatory, anti- hyperglycemic, anti-cancer and anti-atherosclerotic activity. The objective of this study was to assess the effect two cytokinins (BAP and Kinetin) on shoot growth and two auxin on callus induction (2, 4-D and NAA). Highest multiple shoots (4.33±0.63) and length of shoot (5.01±0.53) were induced from nodal explants on modified MS medium supplemented with the 1.0 mg/L 6-benzyadenine and 3%. Among the two auxin used for the investigation leave explants showed highest callus induction in MS media supplemente with 1.0mg/L 2,4-d