Ezrin Binds to DEAD-Box RNA Helicase DDX3 and Regulates Its Function and Protein Level

Abstract

Ezrin is a key regulator of cancer metastasis that links extracellular matrix to the actin cytoskeleton and regulates cell morphology and motility. We discovered a small molecule inhibitor, NSC305787 that directly binds to ezrin and inhibits its function. In this study, we used a nano LC-MS/MS-based proteomic approach to identify ezrin-interacting proteins that are competed away by NSC305787. A large number of the proteins that interact with ezrin were implicated in protein translation and stress granule dynamics. We validated direct interaction between ezrin and the RNA helicase DDX3, and NSC305787 blocked this interaction. Down-regulation or long-term pharmacological inhibition of ezrin led to reduced DDX3 protein levels without changes in DDX3 mRNA. Ectopic overexpression of ezrin in low ezrin-expressing osteosarcoma cells caused a notable increase in DDX3 protein levels. Ezrin inhibited RNA helicase activity of DDX3 but increased its ATPase activity. Our data suggest that ezrin controls the translation of mRNAs preferentially with a structured 5' -untranslated region, at least in part, by sustaining the protein level of DDX3 and/or regulating its function. Therefore, our findings suggest a novel function for ezrin in regulation of gene translation that is distinct from its canonical role as a cytoskeletal scaffold at the cell membrane.