Long non-coding RNA MIR4435-2HG promotes the progression of head and neck squamous cell carcinoma by regulating the miR-383-5p/RBM3 axis

Abstract

Recent studies have shown that long non-coding RNAs (lncRNAs) are strongly related to the progression of various types of cancer. The lncRNA MIR4435-2 host gene (MIR4435-2HG) has been recently recognized as a tumor-related lncRNA that is upregulated in several tumors. However, its possible functions in head and neck squamous cell carcinoma (HNSCC) remain unclear. In tShe present study, we observed that MIR4435-2HG expression was markedly upreg- ulated in HNSCC tissues based on a Gene Expression Profiling Interactive Analysis dataset. This result was further confirmed in HNSCC tissues and cell lines using quantitative real-time polymerase chain reaction. In addition, the high expression level of MIR4435-2HG was significantly associated with poor disease-free survival and overall survival in all HNSCC cases and was associated with advanced tumor-metastasis-node stage and poor prognosis. In vitro and in vivo assays demon- strated that MIR4435-2HG knockdown suppressed HNSCC cell proliferation and invasion, epithelial-mesenchymal transition (EMT), and tumor growth as determined by Cell Counting Kit-8, Transwell assays and western blotting. Furthermore, MIR4435-2HG affected HNSCC cell prolifera- tion and migration and EMT by modulating the microRNA miR-383-5p to positively regulate the protein expression level of RNA-binding motif protein 3 (RBM3). In conclusion, we provide a detailed analysis of the roles of MIR4435-2HG in HNSCC and identified the MIR4435-2HG/miR-383-5p/RBM3 axis as a potential therapeutic target for HNSCC treatment.