PCR primers targeting cis-acting promoter elements in plants

Abstract

The cis-acting elements present in gene promoters are important for promoter function. Thermal Asymmetric Interlaced PCR (TAIL-PCR) provides a simple means for isolating promoter sequences, but the necessary primers can be troublesome to design. Here, we describe an approach, which targets cis-acting elements for TAIL-PCR. The method combines the advantages of TAIL-PCR and SiteFinding-PCR. The new method proved successful for isolating a number of plant promoter sequences. © 2012 Pleiades Publishing, Ltd.