Effects Elecitors on Prophenonoloxidase and Superoxide Anion Activities of Freshwater Prawn, Macrobrachium malcolmsonii

Abstract

The prophenoloxidase and superoxide anion activity of haemocyte lysate supernatant (HLS) from Indian River Prawn (Macrobrachium malcolmsonii) were examined by treating HLS with various factors such as an increase in temperature from 25 to 60�C and elicitors such as ß-1,3-1, 6-glucan, zymosan, levamisole, vitamin C, vitamin E and trypsin. The strongest PO activity was induced at 50�C followed by 45�C. The glucan, zymosan, vitamin C and vitamin E showed higher HLS prophenolase activity, whereas levamisole and trypsin showed lower HLS prophenolase activity relative to the control. In vitro elicitor zymosan showed the maximum percentage of average relative PO activity (272.0%) followed by glucan (215.0%). Levamisole showed maximum percentage elevation (33.4%) of superoxide anion, followed by glucan (25.98%), vitamin E (16.34%), vitamin C (14.87%), trypsin (9.56%) and zymosan (7.0%) respectively.