Direct and indirect effects of elevated CO 2 are revealed through shifts in phytoplankton, copepod development, and fatty acid accumulation

Abstract

Change in the nutritional quality of phytoplankton is a key mechanism through which ocean acidification can affect the function of marine ecosystems. Copepods play an important role transferring energy from phytoplankton to higher trophic levels, including fatty acids (FA)—essential macronutrients synthesized by primary producers that can limit zooplankton and fisheries production. We investigated the direct effects of pCO 2 on phytoplankton and copepods in the laboratory, as well as the trophic transfer of effects of pCO 2 on food quality. The marine cryptophyte Rhodomonas salina was cultured at 400, 800, and 1200 μatm pCO 2 and fed to adult Acartia hudsonica acclimated to the same pCO 2 levels. We examined changes in phytoplankton growth rate, cell size, carbon content, and FA content, and copepod FA content, grazing, respiration, egg production, hatching, and naupliar development. This single-factor experiment was repeated at 12C and at 17C. At 17C, the FA content of R. salina responded non-linearly to elevated pCO 2 with the greatest FA content at intermediate levels, which was mirrored in A. hudsonica; however, differences in ingestion rate indicate that copepods accumulated FA less efficiently at elevated pCO 2 . A. hudsonica nauplii developed faster at elevated pCO 2 at 12C in the absence of strong food quality effects, but not at 17C when food quality varied among treatments. Our results demonstrate that changes to the nutritional quality of phytoplankton are not directly translated to their grazers, and that studies that include trophic links are key to unraveling how ocean acidification will drive changes in marine food webs.