Method of quantitative analysis by HPLC and confirmation by lc-ms/ms of erythritol, maltitol, lactitol and trehalose in foods

Abstract

A quantitative determination method of erythritol, maltitol, lactitol and trehalose in foods by HPLC, and confirmation method by LC-MS/MS were developed. HPLC analysis was performed on a separation column packed with amino group-binding polymer with acetonitrile-water（80: 20）as the mobile phase. The column was operated at room temperature, and the three sugar alcohols and trehalose were quantified. LC-MS/MS confirmation was performed on an amino group-bound column with acetonitrile-ammonium acetate solution as the mobile phase, with detection in the SRM mode. At low sample dilution ratios, the analysis may be affected by matrix derived from the sample, but this can be suppressed by 1,000-fold or greater dilution. Recoveries of the three sugar alcohols and trehalose spiked into food samples, such as tea, jelly, tablets（ramune candy）, and chocolate, exceeded 90％（CV≦6.1％）in HPLC and 94％（CV≦4.8％）in LC-MS/MS.